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PE anti-mouse CD16/32 Antibody
产品名称：
PE anti-mouse CD16/32 Antibody
产品类别：
抗体
产品编号：
101307
产品应用：
101307
[价格]
规格 价格 库存
50µg ￥ 1176 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sorted pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.

The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 ?g per 106 cells in 100 ?L volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered).

Application References

(PubMed link indicates BioLegend citation)
  1. Personal communication (IP)
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  4. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block)
  5. Clements JL, et al. 2006. J. Immunol. 177:905.
  6. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed
  7. Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed
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  9. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
  10. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  11. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
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  13. Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed
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Product Citations
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  3. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
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  6. Chen L, et al. 2022. J Nanobiotechnology. 20:210. PubMed
  7. Xiao S, et al. 2008. J Immunol. 180:914. PubMed
  8. Sun Y et al. 2018. Cancer cell. 34(4):643-658 . PubMed
  9. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  10. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
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  12. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  13. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  14. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  15. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  16. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  17. Amo-Aparicio J, et al. 2021. Theranostics. 11:9805. PubMed
  18. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  19. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  20. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  21. Dong S, et al. 2021. Nature. 591:117. PubMed
  22. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
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RRID
AB_312806 (BioLegend Cat. No. 101307) AB_312807 (BioLegend Cat. No. 101308)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355.
3. Qiu WQ, et al. 1990. Science 248:732.

Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org

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