PE anti-mouse CD317 (BST2, PDCA-1) Antibody
- 产品名称：
- PE anti-mouse CD317 (BST2, PDCA-1) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 127009
- 产品应用：
- 127009
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse plasmacytoid dendritic cells (DCs)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Blasius AL, et al. 2006. J. Immunol. 177:3260.
- Schliemann C, et al. 2010. Blood 115:736. (FA, IF)
- Rajagopal D, et al. 2010. Blood 115:1949. (Depletion)
- Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion)
- Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed
- Product Citations
-
- Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
- de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
- ?lvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
- Toivonen R, et al. 2016. J Immunol. 196: 4750 - 4759. PubMed
- Kimura T, et al. 2020. Commun Biol. 0.527777778. PubMed
- Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
- Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
- Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
- Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
- Lee A, et al. 2022. Nat Commun. 13:549. PubMed
- RRID
- AB_1953284 (BioLegend Cat. No. 127009) AB_1953285 (BioLegend Cat. No. 127010)
- Structure
- Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
- Distribution
-
Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.
- Function
- Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
- Cell Type
- Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Douglas JL. et al. 2009. J Virol. 83(16):7931
2. Cao W et al. 2009. J. Exp. Med. 206(7):1603
3. Neil SJ. et al. 2008. Nature 451:425
- Gene ID
- 69550 View all products for this Gene ID
- UniProt
- View information about CD317 on UniProt.org
