Alexa Fluor® 488 anti-mouse CD317 (BST2, PDCA-
- 产品名称：
- Alexa Fluor® 488 anti-mouse CD317 (BST2, PDCA-
- 产品类别：
- 抗体
- 产品编号：
- 127012
- 产品应用：
- 127012
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse plasmacytoid dendritic cells (DCs)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Blasius AL, et al. 2006. J. Immunol. 177:3260.
- Schliemann C, et al. 2010. Blood 115:736. (FA, IF)
- Rajagopal D, et al. 2010. Blood 115:1949. (Depletion)
- Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion)
- Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed
- Product Citations
-
- Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
- Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
- Xiao Y, et al. 2021. Cell. 184:6037. PubMed
- Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
- Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
- Kim H, et al. 2015. Cytotherapy. . PubMed
- RRID
- AB_1953287 (BioLegend Cat. No. 127012)
- Structure
- Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
- Distribution
-
Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.
- Function
- Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
- Cell Type
- Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Douglas JL. et al. 2009. J Virol. 83(16):7931
2. Cao W et al. 2009. J. Exp. Med. 206(7):1603
3. Neil SJ. et al. 2008. Nature 451:425
- Gene ID
- 69550 View all products for this Gene ID
- UniProt
- View information about CD317 on UniProt.org
