Biotin anti-human CD115 (CSF-1R) Antibody
- 产品名称:
- Biotin anti-human CD115 (CSF-1R) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 347314
- 产品应用:
- 347314
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- C-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature.? Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.?Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151.
- Application References
(PubMed link indicates BioLegend citation) -
- Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
- RRID
- AB_2650968 (BioLegend Cat. No. 347314)
- Structure
- Single-pass type I membrane protein and is 150 kD.
- Distribution
-
Monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts.
- Ligand/Receptor
- Macrophage Colony Stimulating Factor (M-CSF) and IL-34.
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Osteoclasts
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sherr CJ, et al. 1989. Blood 73:1786
2. Roussel MF, et al. 1991. Nature 353:361.
3. Roussel MF, et al. 1989 P. Natl. Acad. Sci. USA 86:7924.
- Gene ID
- 1436 View all products for this Gene ID
- UniProt
- View information about CD115 on UniProt.org