Alexa Fluor® 647 anti-human CD115 (CSF-1R) Ant
- 产品名称：
- Alexa Fluor® 647 anti-human CD115 (CSF-1R) Ant
- 产品类别：
- 抗体
- 产品编号：
- 347315
- 产品应用：
- 347315
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1836 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- C-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature.? Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.?Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151.
- Application References
(PubMed link indicates BioLegend citation) -
- Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
- RRID
- AB_2687249 (BioLegend Cat. No. 347315) AB_2687250 (BioLegend Cat. No. 347316)
- Structure
- Single-pass type I membrane protein and is 150 kD.
- Distribution
-
Monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts.
- Ligand/Receptor
- Macrophage Colony Stimulating Factor (M-CSF) and IL-34.
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Osteoclasts
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sherr CJ, et al. 1989. Blood 73:1786
2. Roussel MF, et al. 1991. Nature 353:361.
3. Roussel MF, et al. 1989 P. Natl. Acad. Sci. USA 86:7924.
- Gene ID
- 1436 View all products for this Gene ID
- UniProt
- View information about CD115 on UniProt.org
