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Purified anti-RNA Polymerase II RPB1 Antibody
产品名称：
Purified anti-RNA Polymerase II RPB1 Antibody
产品类别：
抗体
产品编号：
664906
产品应用：
664906
[价格]
规格 价格 库存
100µg ￥ 3876 1

产品详情

Product Details

Verified Reactivity
Human, Yeast, Wheat, Mouse, C. elegans, X. laevis
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Wheat germ RNA Polymerase II
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ChIP - Verified
FA, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 ?g per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL?is recommended.?The suggested dilution for ChIP application is 1:300 - 1:500?by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 8WG16 recognizes YSPTSPS in the C-terminal domain. The antibody cross-reacts with wheat, yeast, mouse, c. elegans, x. laevis, and most eukaryotic RNAPII.

Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation, functional assay, and immunoprecipatation.

Application References

(PubMed link indicates BioLegend citation)
  1. Edwards AM, et al. 1990. Proc. Natl. Acad. Sci. USA. 87:2122.
  2. Thompson NE, et al. 1990. J. Biol. Chem. 265:7069.
  3. Thompson NE, et al. 1989. J. Biol. Chem. 264:11511.
Product Citations
  1. Niu L, et al. 2021. Nat Genet. 53:1075. PubMed
  2. Onishi R, et al. 2020. Sci Adv. 6:00. PubMed
  3. Edwards SL, et al. 2021. Cell Reports. 36(9):109623. PubMed
  4. Catala M, et al. 2019. Commun Biol. 2:211. PubMed
  5. Zhao X, et al. 2022. Nat Commun. 13:5401. PubMed
  6. Wang Y, et al. 2020. J Biol Chem. . PubMed
  7. Di Giammartino DC, et al. 2022. Methods Mol Biol. 2532:113. PubMed
  8. Huang Z, et al. 2021. Molecular Cell. 81(5):953-968.e9. PubMed
  9. Montinaro A, et al. 2021. Cell Death Differ. Online ahead of print. PubMed
  10. Legrand N et al. 2019. Nucleic Acids Res. 47(18):9573-9591 . PubMed
  11. Paakinaho V, et al. 2021. Nucleic Acids Res. 49:1951. PubMed
  12. Das S, et al. 2020. eLife. 9:00. PubMed
  13. Rowley MJ, et al. 2019. Cell Rep. 26:2890. PubMed
  14. Cardoso da Silva R, et al. 2020. PLoS Genet. 16:e1008905. PubMed
  15. Lee J et al. 2018. Cell stem cell. 22(3):428-444 . PubMed
  16. Vadivel CK, et al. 2021. Cancers (Basel). 13:00. PubMed
  17. Joo Y, et al. 2020. Nat Commun. 2.640972222. PubMed
  18. Rhie SK, et al. 2018. Genome Res. 13:e0196698. PubMed
  19. Fan X, et al. 2021. PLoS Genet. 17:e1009773. PubMed
  20. Thouvenot P, et al. 2016. J Cell Sci. 129(23):4366-4378. PubMed
  21. Yu Y, et al. 2016. Sci Rep. 6:31752. PubMed
  22. See YX, et al. 2022. Genome Res. . PubMed
RRID
AB_2565554 (BioLegend Cat. No. 664906)

Antigen Details

Structure
RPB1 is a 220 kD subunit of RNA polymerase II and has 1970 amino acids.
Distribution

Nucleus; ubiquitous expressed in all cells

Function
Core element of RNA polymerase II transcription machinery.
Interaction
COMPASS (COMplex of Proteins ASsociated with Set1), SET2, BRE1
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Chávez S,?et al.?2016. CUrr. Genet. 62:701.
Gene ID
5430 View all products for this Gene ID 20020 View all products for this Gene ID 2540292 View all products for this Gene ID
UniProt
View information about RNA Polymerase II RPB1 on UniProt.org

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