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Alexa Fluor® 647 anti-RNA Polymerase II RPB1 A
产品名称：
Alexa Fluor® 647 anti-RNA Polymerase II RPB1 A
产品类别：
抗体
产品编号：
664913
产品应用：
664913
[价格]
规格 价格 库存
25µg ￥ 1908 1

产品详情

Product Details

Verified Reactivity
Human, Yeast, Wheat, Mouse, C. elegans, X. laevis
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Wheat germ RNA Polymerase II
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

?

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 8WG16 recognizes YSPTSPS in the C-terminal domain. The antibody cross-reacts with wheat, yeast, mouse, c. elegans, x. laevis, and most eukaryotic RNAPII.

Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation, functional assay, and immunoprecipatation.

Application References

(PubMed link indicates BioLegend citation)
  1. Edwards AM, et al. 1990. Proc. Natl. Acad. Sci. USA. 87:2122.
  2. Thompson NE, et al. 1990. J. Biol. Chem. 265:7069.
  3. Thompson NE, et al. 1989. J. Biol. Chem. 264:11511.
RRID
AB_2728497 (BioLegend Cat. No. 664913) AB_2728498 (BioLegend Cat. No. 664914)

Antigen Details

Structure
RPB1 is a 220 kD subunit of RNA polymerase II and has 1970 amino acids.
Distribution

Nucleus; ubiquitous expressed in all cells

Function
Core element of RNA polymerase II transcription machinery.
Interaction
COMPASS (COMplex of Proteins ASsociated with Set1), SET2, BRE1
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Chávez S,?et al.?2016. CUrr. Genet. 62:701.
Gene ID
5430 View all products for this Gene ID 20020 View all products for this Gene ID 2540292 View all products for this Gene ID
UniProt
View information about RNA Polymerase II RPB1 on UniProt.org

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