Purified anti--上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3876	1

Product Details

Verified Reactivity

E. coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Purified recombinant E. coli RNA polymerase β subunit.

Formulation

0.22 ?m filtered protein solution is in phosphate-buffered solution, pH 7.2.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. May be sub-aliquoted and stored at -20°C to avoid multiple freeze thaw cycles.

Application

WB - Quality tested
ELISA, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.

Additional reported applications (for the relevant formats) include: immunoprecipitation³.?The epitope of clone 8RB13 has been mapped to the sequence?PEEKLLRAIFGEKAS³.

Application References (PubMed link indicates BioLegend citation)

Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
Stalder, ES.?et al.?2011.?Protein Expr. Purif.?77(1):26-33. (Epitope, ELISA, IP, WB)

Product Citations

d'Andrea FB, et al. 2022. Sci Adv. 8:eabn7943. PubMed
Dupuy P, et al. 2020. Proc Natl Acad Sci U S A. 117:19517. PubMed
Liang X, et al. 2021. PLoS Pathog. 17:e1010116. PubMed
Wipperman MF et al. 2018. Molecular cell. 72(1):152-161 . PubMed
Olivencia B,et al. 2017. Sci Rep.. 10.1038/s41598-017-14410-z. PubMed
H?r J, et al. 2020. Nucleic Acids Res. 48:9301. PubMed
Dupuy P, et al. 2022. Nat Commun. 13:4493. PubMed
Liang X, et al. 2021. Appl Environ Microbiol. 87:e0043521. PubMed

RRID

AB_2566583 (BioLegend Cat. No. 663905)

Antigen Details

Structure: 1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution: Bacteria.
Function: Transcriptional regulator in bacteria.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
Gene ID: 948488 View all products for this Gene ID
UniProt: View information about RNA Polymerase beta on UniProt.org

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