Direct-Blot™ HRP anti--上海雅吉生物科技有限公司

规格	价格	库存
100µL	￥ 4080	1

Product Details

Verified Reactivity

E. coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Purified recombinant E. coli RNA polymerase β subunit.

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.

Additional reported applications (for the relevant formats) include: immunoprecipitation³.?The epitope of clone 8RB13 has been mapped to the sequence?PEEKLLRAIFGEKAS³.

Application References (PubMed link indicates BioLegend citation)

Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
Stalder, ES.?et al.?2011.?Protein Expr. Purif.?77(1):26-33. (Epitope, ELISA, IP, WB)

RRID

AB_2629625 (BioLegend Cat. No. 663907)

Antigen Details

Structure: 1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution: Bacteria.
Function: Transcriptional regulator in bacteria.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
Gene ID: 948488 View all products for this Gene ID
UniProt: View information about RNA Polymerase beta on UniProt.org

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