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首页 > 抗体蛋白 > 抗体
Anti-
产品名称：
Anti-
产品类别：
抗体
产品编号：
663903
产品应用：
663903
[价格]
规格 价格 库存
100µL ￥ 3876 1

产品详情

Product Details

Verified Reactivity
E. coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant E. coli RNA polymerase β subunit.
Preparation
Dried ascites
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Store lyophilized antibody at -20°C. Avoid repeat freeze/thaw cycles.
Application

WB - Quality tested
ELISA - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 ?l of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconsitution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.

Additional reported applications (for the relevant formats) include: immunoprecipitation3.?The epitope of clone 8RB13 has been mapped to the sequence?PEEKLLRAIFGEKAS3.

Application References

(PubMed link indicates BioLegend citation)
  1. Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
  2. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
  3. Stalder, ES.?et al.?2011.?Protein Expr. Purif.?77(1):26-33. (Epitope, ELISA, IP, WB)
Product Citations
  1. Heinrich K, et al. 2019. MBio. 10. PubMed
  2. Ju X, et al. 2019. Nat Microbiol. 1.490972222. PubMed
  3. Broder U, Jaeger T, Jenal U 2016. Nat Microbiol. 2:16184. PubMed
  4. Ting SY, et al. 2020. Cell Host Microbe. 28(2):313-321.e6. PubMed
  5. Ting SY, et al. 2022. Elife. 11:. PubMed
  6. Kouba T, et al. 2020. Nat Commun. 11:6419. PubMed
  7. Stringer AM, et al. 2020. Elife. 9:00. PubMed
  8. Icke C, et al. 2021. eLife. 10:00. PubMed
  9. Osuna BA, et al. 2020. Cell Host Microbe. 28:31. PubMed
  10. Pribis JP et al. 2019. Mol Cell. 74(4):785-800 . PubMed
RRID
AB_2564524 (BioLegend Cat. No. 663903)

Antigen Details

Structure
1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution

Bacteria.

Function
Transcriptional regulator in bacteria.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.

Gene ID
948488 View all products for this Gene ID
UniProt
View information about RNA Polymerase beta on UniProt.org

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