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Alexa Fluor® 647 anti-mouse IL-17F Antibody
产品名称:
Alexa Fluor® 647 anti-mouse IL-17F Antibody
产品类别:
抗体
产品编号:
517104
产品应用:
517104
[价格]
规格 价格 库存
100µg ¥ 4368 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse IL-17F-OVA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA Detection: The biotinylated 8F5.1A9 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10.1 (Cat. No. 506901 or 506906) antibody as the capture antibody and recombinant mouse IL-17A/F (Cat. No. 580809) as the standard.

Flow Cytometry: The fluorochrome-labeled 8F5.1A9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17F-producing cells within mixed cell populations.

Note: For testing mouse IL-17F in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX? Kits (Cat. No. 436107 & 436108) are specially developed and recommended.

RRID
AB_2125412 (BioLegend Cat. No. 517104)

Antigen Details

Structure
Forms a 37 kD homodimer or heterodimer with IL-17A, a IL-17 family member.
Distribution
Th17 cells, mast cells, basophils, and epithelial cells.
Function
An important regulator of inflammatory responses, involved in cancer immunity and autoimmune responses as well.
Ligand/Receptor
IL-17R (CD217)
Cell Type
Th17, Mast cells, Basophils, Epithelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Dong C. 2008. Immunol. Rev. 226:80.
2. Kolls JK, et al. 2004. Immunity 21:467.
3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1.
4. Yang XO, et al. 2008. J. Exp. Med. 205:1063.
5. Ishigame H, et al. 2009. Immunity 30:108.
6. Hu Y, et al. 2010. J. Immunol. 184:4307.

Gene ID
257630 View all products for this Gene ID
UniProt
View information about IL-17F on UniProt.org

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