Purified anti-human IL-4 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 996	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Yeast-expressed, recombinant human IL-4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, ICFC, IHC, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The purified 8D4-8 has been tested by blocking fluorochrome conjugated 8D4-8 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (≤ 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISPOT Capture, neutralization, intracellular staining for flow cytometry, immunohistochemical staining, and immunoprecipitation.

Application References (PubMed link indicates BioLegend citation)

Bird, C., et al. 1991. Cytokine. 3:562.
Prussin, C., et al. 1995. J. Immunol. Meth. 188:117.
Hickie, I., et al. 2001. Twin Res. 4:94.
Heeger, P., et al. 1999. J. Immunol. 163:2267.
Jason, J., et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Gong, G, et al. 2009. Blood 113:837. PubMed
Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

Product Citations

Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed
Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed
Kameda M, et al. 2020. PLoS One. 15:e0241719. PubMed
Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
Kurtaj A, et al. 2017. J Allergy Clin Immunol. 140:1746. PubMed

RRID

AB_315113 (BioLegend Cat. No. 500701) AB_315114 (BioLegend Cat. No. 500702)

Antigen Details

Structure: Cytokine; 15-19 kD (Mammalian)
Bioactivity: Differentiation of na?ve CD4⁺ T cells to the T_H2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors: Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Boulay, J., et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens, H., et al. 1991. In vivo 5:567.
4. Paul, W. 1991. Blood. 77:1859.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 3565 View all products for this Gene ID
UniProt: View information about IL-4 on UniProt.org

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