PerCP/Cyanine5.5 anti-human CD325 (N-Cadherin) Ant-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1764	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human N-cadherin extracellular domain

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The mAb 8C11 recognizes the amino acids 92–593 of CD325, located between the extracellular cadherin structural domain (EC) 3 and 4. Additional reported applications (for the relevant formats) include: immunofluorescence^1,3,6, motility inhibition of N-cadherin-expressing cells², and Western blot^2,4.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Navarro P, et al. 1998. J. Cell Biol. 140:1475. (IF)
Kim JB. 2000. J. Cell Biol. 151:1193. (Block, WB)
Puch S, et al. 2001. J. Cell. Sci. 114:1567. (IF)
Wahl JK. 3rd, et al. 2003. J. Biol. Chem. 278:17269. (WB)
Wein F, et al. 2010. Stem. Cell Res. 4:129. (FC)
Jaggi M, et al. 2002. Cell. Commun. Adhes. 9:103. (IF)

Product Citations

Chirshev E, et al. 2020. Mol Oncol. . PubMed

RRID

AB_2562685 (BioLegend Cat. No. 350813) AB_2562686 (BioLegend Cat. No. 350814)

Antigen Details

Structure: Member of cadherin family, Single pass transmembrane protein, extracellular region consist of five EC domains, one cytoplasmic domain, 130 kD
Distribution: Stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, some types of carcinoma cells
Function: Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility
Interaction: Catenins
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Colomiere M, et al. 2009. Brit. J. Cancer 100:134.
2. Yan W, et al. 2010. J. Biol. Chem. 285:14042.
3. Mosnier JF, et al. 2009. Mod. Pathol. 22:182.
4. Gao L, et al. 2010. Stem Cells 28:564.
Gene ID: 1000 View all products for this Gene ID
UniProt: View information about CD325 on UniProt.org

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