Alexa Fluor® 594 anti-human CD325 (N-Cadherin)-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3876	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human N-cadherin extracellular domain

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

The mAb 8C11 recognizes the amino acids 92–593 of CD325, located between the extracellular cadherin structural domain (EC) 3 and 4. Additional reported applications (for the relevant formats) include: immunofluorescence^1,3,6, motility inhibition of N-cadherin-expressing cells², and Western blot^2,4.

Application References (PubMed link indicates BioLegend citation)

Navarro P, et al. 1998. J. Cell Biol. 140:1475. (IF)
Kim JB. 2000. J. Cell Biol. 151:1193. (Block, WB)
Puch S, et al. 2001. J. Cell. Sci. 114:1567. (IF)
Wahl JK. 3rd, et al. 2003. J. Biol. Chem. 278:17269. (WB)
Wein F, et al. 2010. Stem. Cell Res. 4:129. (FC)
Jaggi M, et al. 2002. Cell. Commun. Adhes. 9:103. (IF)

Product Citations

Aboulkheyr Es H, et al. 2021. J Cell Physiol. 236:3918. PubMed

RRID

AB_2563226 (BioLegend Cat. No. 350816)

Antigen Details

Structure: Member of cadherin family, Single pass transmembrane protein, extracellular region consist of five EC domains, one cytoplasmic domain, 130 kD
Distribution: Stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, some types of carcinoma cells
Function: Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility
Interaction: Catenins
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Colomiere M, et al. 2009. Brit. J. Cancer 100:134.
2. Yan W, et al. 2010. J. Biol. Chem. 285:14042.
3. Mosnier JF, et al. 2009. Mod. Pathol. 22:182.
4. Gao L, et al. 2010. Stem Cells 28:564.
Gene ID: 1000 View all products for this Gene ID
UniProt: View information about CD325 on UniProt.org

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