Ultra-LEAF™ Purified anti-Histone Lysine Dem
- 产品名称：
- Ultra-LEAF™ Purified anti-Histone Lysine Dem
- 产品类别：
- 抗体
- 产品编号：
- 844903
- 产品应用：
- 844903
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein consisting of NO66 protein fragment (aa 423-427) and Hepatitis B virus capsid protein.
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2566003 (BioLegend Cat. No. 844903)
- Structure
- 641 amino acids; 71 kD.
- Distribution
- Nucleus, nucleolus, and nucleoplasm.
- Function
- Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and momomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216' and acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (by similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.
- Biology Area
- Cell Biology, Neuroscience
- Antigen References
-
1. Vasilatos S, et al. 2013. Carcinogenesis 34:1196.
2. Iwamori N, et al. 2011. Biol. Reprod. 84:1225.
3. Tao Y, et al. 2013. J. Biol. Chem. 288:16430.
- Gene ID
- 79697 View all products for this Gene ID
- UniProt
- View information about NO66 on UniProt.org
