Alexa Fluor® 488 anti-human HLA-G Antibody
- 产品名称：
- Alexa Fluor® 488 anti-human HLA-G Antibody
- 产品类别：
- 抗体
- 产品编号：
- 335917
- 产品应用：
- 335917
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1836 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HLA-G transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
87G mAb reacts with isoforms of HLA-G1 and -G5. Additional reported applications (for the relevant formats) include: restoring HLA-G mediated suppression of allo-T cell proliferation and immunohistochemical staining of frozen tissue sections. Clone 87G is reported not to bind HLA-G in aldehyde fixed samples.4
- Application References
(PubMed link indicates BioLegend citation) -
- Odum N, et al. 1991. Eur. J. Immunol. 21:2121.
- Lila N, et al. 2001. P. Natl. Acad. Sci. USA 98:12150.
- Lila N, et al. 2002. Circulation. 105:1949.
- Blaschitz A, et al. 2000. Hum. Immunol. 61:1074.
- Balsamo M, et al. 2012. Eur J. Immunol. 42:1833. PubMed
- Product Citations
-
- Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
- RRID
- AB_2728295 (BioLegend Cat. No. 335917) AB_2728296 (BioLegend Cat. No. 335918)
- Structure
- Ig
- Distribution
-
Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, various tumors
- Function
- Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8+ T cells, induce Th2 cytokine production
- Ligand/Receptor
- ILT2(CD85j), ILT4(CD85d), KIR2DL4(CD158d), CD8α/α
- Cell Type
- Dendritic cells, Epithelial cells, Macrophages, Monocytes
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Hunt JS, et al. 2005. FASEB J. 19:681.
2. Carosella ED, et al. 2008. Blood 111:4862.
- Gene ID
- 3135 View all products for this Gene ID
- UniProt
- View information about HLA-G on UniProt.org
