PE anti-mouse Podoplanin Antibody
- 产品名称：
- PE anti-mouse Podoplanin Antibody
- 产品类别：
- 抗体
- 产品编号：
- 127408
- 产品应用：
- 127408
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.
- Application References
(PubMed link indicates BioLegend citation) -
- Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
- Farr AG, et al. 1992. J. Exp. Med. 176:1477.
- Bekiaris V, et al. 2008. J. Immunol. 180:6768.
- Algars A, et al. 2011. Blood 117:4387. PubMed
- Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
- Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
- Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Krausgruber T, et al. 2020. Nature. 583:296. PubMed
- Zinselmeyer B, et al. 2013. J Exp Med. 210:757. PubMed
- Luo R, et al. 2021. Theranostics. 11:117. PubMed
- Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
- Novkovic M, et al. 2020. Cell Reports. 30(3):893-904.e6.. PubMed
- Chauhan A, et al. 2020. Nat Commun. 11:1939. PubMed
- Liu M, et al. 2020. Nature. 587:115. PubMed
- Mkrtichyan M, et al. 2012. J Immunol. 189:2450. PubMed
- Hara T, et al. 2012. J Immunol. 189:1577. PubMed
- Emg?rd J, et al. 2018. Immunity. 143:419. PubMed
- Bresson L, et al. 2018. Development. 145. PubMed
- Sun Q, et al. 2015. Bone Res. 3: 15026. PubMed
- Sullivan B, et al. 2015. PLoS Pathog. 11:1004588. PubMed
- Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
- Progatzky F, et al. 2021. Nature. 599:125. PubMed
- Pezoldt J, et al. 2022. Nat Commun. 13:7227. PubMed
- Baasch S, et al. 2021. Cell. . PubMed
- Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
- Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
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- Tang C, et al. 2021. Theranostics. 11:9791. PubMed
- Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed
- Ito K, et al. 2014. Proc Natl Acad Sci U S A. 111:3080. PubMed
- Karpus ON, et al. 2019. Cell Rep. 26:3698. PubMed
- Francipane MG, et al. 2020. Am J Pathol. 190:252. PubMed
- Kataru RP, et al. 2022. Front Aging. 3:864860. PubMed
- Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
- Mishra A, et al. 2016. J Immunol. 197: 2828 - 2837. PubMed
- Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
- Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
- Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
- Goldberg AR, et al. 2022. Front Physiol. 13:1021038. PubMed
- Bieniasz-Krzywiec P, et al. 2021. STAR Protoc. 2:100481. PubMed
- Othy S, et al. 2013. J Immunol. 190:4535. PubMed
- Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
- RRID
- AB_2161929 (BioLegend Cat. No. 127407) AB_2161928 (BioLegend Cat. No. 127408)
- Structure
- 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
- Distribution
-
Expressed on epithelial and mesothelial cells.
- Cell Type
- Epithelial cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.
- Gene ID
- 14726 View all products for this Gene ID
- UniProt
- View information about Podoplanin on UniProt.org
