Alexa Fluor® 488 anti-mouse Podoplanin Antibod
- 产品名称：
- Alexa Fluor® 488 anti-mouse Podoplanin Antibod
- 产品类别：
- 抗体
- 产品编号：
- 127405
- 产品应用：
- 127405
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.
- Application References
(PubMed link indicates BioLegend citation) -
- Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
- Farr AG, et al. 1992. J. Exp. Med. 176:1477.
- Bekiaris V, et al. 2008. J. Immunol. 180:6768.
- Algars A, et al. 2011. Blood 117:4387. PubMed
- Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
- Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
- Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
- Hoorweg K, et al. 2015. J Immunol. 195: 4257 - 4263. PubMed
- Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
- Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
- Fujikura D, et al. 2013. PLoS One. 8:e55321. PubMed
- Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
- Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
- Nadafi R, et al. 2020. Cell Reports. 30(12):4110-4123. PubMed
- Jung M, et al. 2016. Sci Signal. 9: ra64. PubMed
- Durré T, et al. 2018. Nat Commun. 9:5178. PubMed
- RRID
- AB_1133992 (BioLegend Cat. No. 127405) AB_2161930 (BioLegend Cat. No. 127406)
- Structure
- 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
- Distribution
-
Expressed on epithelial and mesothelial cells.
- Cell Type
- Epithelial cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.
- Gene ID
- 14726 View all products for this Gene ID
- UniProt
- View information about Podoplanin on UniProt.org
