PerCP/Cyanine5.5 anti-mouse CD21/CD35 (CR2/CR1) An
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD21/CD35 (CR2/CR1) An
- 产品类别：
- 抗体
- 产品编号：
- 123415
- 产品应用：
- 123415
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CD35/CFA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Boackle S, et al. 2001 Immunity 15:775.
- de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
- Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
- Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Gawish R, et al. 2022. Elife. 11:. PubMed
- Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
- Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
- Manzl C, et al. 2012. Cell Death Differ. 18:54. PubMed
- Na B, et al. 2016. PLoS One. 11: 0156429. PubMed
- Lehrke MJ, et al. 2021. Elife. 10:. PubMed
- Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
- Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
- Steinel N, et al. 2014. J Immunol. 193:2881. PubMed
- Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
- Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
- Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
- RRID
- AB_1595595 (BioLegend Cat. No. 123415) AB_1595490 (BioLegend Cat. No. 123416)
- Structure
- 145 kD/190 kD type I transmembrane glycoprotein
- Distribution
-
B cells, follicular dendritic cells
- Function
- Signal transduction
- Ligand/Receptor
- Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
- Gene ID
- 12902 View all products for this Gene ID 12946 View all products for this Gene ID
- UniProt
- View information about CD21/35 on UniProt.org
