Pacific Blue™ anti-mouse CD21/CD35 (CR2/CR1)
- 产品名称：
- Pacific Blue™ anti-mouse CD21/CD35 (CR2/CR1)
- 产品类别：
- 抗体
- 产品编号：
- 123413
- 产品应用：
- 123413
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CD35/CFA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported application (for relevant formats) include: spatial biology (IBEX)5,6.
- Additional Product Notes
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
- Application References
(PubMed link indicates BioLegend citation) -
- Boackle S, et al. 2001 Immunity 15:775.
- de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
- Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
- Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Bhattacharya Y 2014. J Exp Med. 211:841. PubMed
- Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
- Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
- Juul-Madsen K, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
- Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
- Avery DT, et al. 2018. J Exp Med. 215:2073. PubMed
- Duan L, et al. 2021. Immunity. 54:2256. PubMed
- Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
- Duong B, et al. 2010. J Exp Med. 207:173. PubMed
- Noviski M, et al. 2018. Elife. 7:e35074. PubMed
- Lofano G, et al. 2015. J Immunol. 195: 1617-1627. PubMed
- Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
- Seeley-Fallen M, et al. 2014. Proc Natl Acad Sci U S A. 111:9881. PubMed
- Radice E, et al. 2020. Cell Reports. 32(5):107951. PubMed
- Gill R, et al. 2017. Toxicol Appl Pharmacol. 330:22. PubMed
- RRID
- AB_2085159 (BioLegend Cat. No. 123413) AB_2085158 (BioLegend Cat. No. 123414)
- Structure
- 145 kD/190 kD type I transmembrane glycoprotein
- Distribution
-
B cells, follicular dendritic cells
- Function
- Signal transduction
- Ligand/Receptor
- Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
- Gene ID
- 12902 View all products for this Gene ID 12946 View all products for this Gene ID
- UniProt
- View information about CD21/35 on UniProt.org
