Alexa Fluor® 594 anti-mouse CD21/CD35 (CR2/CR1
- 产品名称：
- Alexa Fluor® 594 anti-mouse CD21/CD35 (CR2/CR1
- 产品类别：
- 抗体
- 产品编号：
- 123426
- 产品应用：
- 123426
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CD35/CFA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-F - Quality tested
3D IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 ?g/mL?is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application References
(PubMed link indicates BioLegend citation) -
- Boackle S, et al. 2001 Immunity 15:775.
- de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
- Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
- Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Nakata T, et al. 2020. Proc Natl Acad Sci U S A. 117:28930. PubMed
- Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
- Bunse M, et al. 2021. Nat Commun. 0.666666667. PubMed
- RRID
- AB_2632698 (BioLegend Cat. No. 123426)
- Structure
- 145 kD/190 kD type I transmembrane glycoprotein
- Distribution
-
B cells, follicular dendritic cells
- Function
- Signal transduction
- Ligand/Receptor
- Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
- Gene ID
- 12902 View all products for this Gene ID 12946 View all products for this Gene ID
- UniProt
- View information about CD21/35 on UniProt.org
