PE anti-mouse CD278 (ICOS) Antibody
- 产品名称：
- PE anti-mouse CD278 (ICOS) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 117405
- 产品应用：
- 117405
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse ICOS cDNA and ICOS hexahistidine fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF? format is suggested for blocking studies.
- Application References
(PubMed link indicates BioLegend citation) -
- Akbari O, et al. 2002. Nat. Med. 8:1024.
- Harada H, et al. 2003. J. Clin. Invest. 112:234.
- McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block)
- Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
- Product Citations
-
- Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
- Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
- Tan X, et al. 2021. Sci Adv. 7: . PubMed
- Yu X, et al. 2019. Nat Commun. 10:574. PubMed
- Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
- Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
- Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed
- Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
- Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
- Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
- RRID
- AB_961244 (BioLegend Cat. No. 117405) AB_2122712 (BioLegend Cat. No. 117406)
- Structure
- CD28/CTLA-4, 47-57kD
- Distribution
-
Activated T cells, subset of thymocytes
- Function
- Costimulates T cell activation, proliferation, humoral immune response
- Ligand/Receptor
- B7h/B7RP-1/GL-50
- Cell Type
- T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
2. McAdam AJ, et al. 2000. J. Immunol. 165:5035.
3. Mak TW, et al. 2003. Nat. Immunol. 4:765
- Gene ID
- 54167 View all products for this Gene ID
- UniProt
- View information about CD278 on UniProt.org
