Purified anti-c-Fos Antibody
- 产品名称：
- Purified anti-c-Fos Antibody
- 产品类别：
- 抗体
- 产品编号：
- 600751
- 产品应用：
- 600751
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant human FOS protein fragments
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICC - Quality tested
ICFC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5?- 10?μg/mL is recommended. For intraceullar flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
For ICC we recommend PFA-MeoH fixation/permeabilization. We do not recommend methanol-only and PFA+ Triton X-100 fixation/permeabilization due to dim signal.
?
For ICFC, we recommend fixation/permeabilization with the True-Phos? Perm Buffer. Cytokine method was tried and failed due to no signal.
- RRID
- AB_2910472 (BioLegend Cat. No. 600751) AB_2910472 (BioLegend Cat. No. 600752)
- Structure
- c-Fos is a 330 amino acid protein with a predicted molecular weight of 41 kD.
- Distribution
-
Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes
- Function
- FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation.
- Interaction
- Interacts with a variety of proteins including c-Jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, Transcription Factors
- Antigen References
-
- Dony C and Gruss P. 1987. Nature.?328:711-4.
- Grigoriadis AE, et al. 1994. Science. 266:443-8.
- Glover JN and Harrison SC. 1995. Nature. 373:257-61.
- Gene ID
- 2353 View all products for this Gene ID
- UniProt
- View information about c-Fos on UniProt.org
