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Purified anti-mouse CD25 Antibody
产品名称:
Purified anti-mouse CD25 Antibody
产品类别:
抗体
产品编号:
154202
产品应用:
154202
[价格]
规格 价格 库存
500µg ¥ 2748 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
HT-2 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, Depletion, IP - Reported in the literature, not verified in house?

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Inhibition of IL-2 dependent proliferation of HT2 cells, immunoprecipitation of CD25 from cell lysates, in vivo depletion of CD25+ cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Malek TR, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:5694. (FC, Block, IP)
  2. Couper KN. 2007. J. Immunol. 178:4136. (Depletion)
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Zhou D, et al. 2022. Nat Commun. 13:5174. PubMed
RRID
AB_2716173 (BioLegend Cat. No. 154202)

Antigen Details

Structure
Type I, 55 kD, transmembrane glycoprotein
Distribution

Activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells

Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Huss DJ, et al. 2015. J. Immunol. 194:84.
2. Hartmann FJ, et al. 2014. Nat. Commun. 5:5056.
3. Codarri L, et al. 2007. J. Exp. Med. 204:1533.
4. Read S, et al. 2000. J. Exp. Med. 192:295.
5. Taniguchi T, et al. 1993. Cell 73:5.?

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

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