Purified anti-mouse CD25 Antibody
- 产品名称:
- Purified anti-mouse CD25 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 154202
- 产品应用:
- 154202
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- HT-2 cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Block, Depletion, IP - Reported in the literature, not verified in house?
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Inhibition of IL-2 dependent proliferation of HT2 cells, immunoprecipitation of CD25 from cell lysates, in vivo depletion of CD25+ cells.
- Application References
(PubMed link indicates BioLegend citation) -
- Malek TR, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:5694. (FC, Block, IP)
- Couper KN. 2007. J. Immunol. 178:4136. (Depletion)
- Product Citations
-
- Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
- Zhou D, et al. 2022. Nat Commun. 13:5174. PubMed
- RRID
- AB_2716173 (BioLegend Cat. No. 154202)
- Structure
- Type I, 55 kD, transmembrane glycoprotein
- Distribution
-
Activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Huss DJ, et al. 2015. J. Immunol. 194:84.
2. Hartmann FJ, et al. 2014. Nat. Commun. 5:5056.
3. Codarri L, et al. 2007. J. Exp. Med. 204:1533.
4. Read S, et al. 2000. J. Exp. Med. 192:295.
5. Taniguchi T, et al. 1993. Cell 73:5.?
- Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org