Purified anti-β-Dystroglycan Antibody
- 产品名称：
- Purified anti-β-Dystroglycan Antibody
- 产品类别：
- 抗体
- 产品编号：
- 850201
- 产品应用：
- 850201
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated peptide corresponding to the last 15 amino acids (KNMTPYRSPPPYVPP) of L-Dystroglycan.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
IHC-F, ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 ?g per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.1 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Praissman JL, et al. 2016. Elife. E14473 (ICC)
- Vasquez-Limeta A, et al. 2014. PLoS One. 9(3):e90629 (WB)
- Martinez-Vieyra IA, et al. 2013. biochim Biophys Acta. 1833(3):698 (ICC, WB)
- Beedle AM, et al. 2012. J Clin Invest. 122(9):3330 (IHC-F, WB)
- Hu H, et al. 2011. Neurosci Lett. 489(1): 10 (WB)
- Product Citations
-
- Dai J, et al. 2022. Nat Cancer. 3:25. PubMed
- RRID
- AB_2715870 (BioLegend Cat. No. 850201) AB_2715871 (BioLegend Cat. No. 850202)
- Structure
- Dystroglycan is an 895 amino acid protein with a molecular mass of ~97 kD. β-dystroglycan, a cleavage product of dystroglycan, is a 242 amino acid protein with a molecular mass of ~43 kD.
- Distribution
-
Tissue distribution: Primarily expressed in muscle tissue. Also present in other tissue including CNS.
Cellular distribution: nucleus, plasma membrane, and cytoskeleton.
- Function
- β-dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
- Interaction
- Interacts with Dystrophin, Utrophin and other cytoskeletal components such as axin.
- Ligand/Receptor
- Receptor for Dystrophin and Utrophin
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
1. Murphy S, et al. 2015. Comput Struct Biotechnol J. 14:20-7
2. Moore CJ, et al. 2012. Neuromuscul Disord. 22(11): 959
- Gene ID
- 1605 View all products for this Gene ID
- UniProt
- View information about beta-Dystroglycan on UniProt.org
