PE/Dazzle™ 594 anti-human/mouse Bcl-6 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1908	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Sequence 226-398 of murine BCL6 fused with GST

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting^1,2,3.

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References (PubMed link indicates BioLegend citation)

Vikstrom, et al. 2010. Science 330:1095. (WB)
Kallies A, et al. 2011. Blood 117:1869. (WB)
Lüthje K, et al. 2012. Nat. Immunol. 13:491. (WB)
Tonti E, et al. 2012. J. Immunol. 188:3217. (ICFC)

RRID

AB_2566193 (BioLegend Cat. No. 358509) AB_2566194 (BioLegend Cat. No. 358510)

Antigen Details

Structure: Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution: Germinal center B cells, follicular helper T cells (T_FH), B cell lymphomas, breast cancer cells
Function: Transcription factor, master regulator of germinal center reaction
Interaction: BLIMP-1
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Basso K and Dalla-Favera R. 2012.?Immunol. Rev.?247:172.
2. Vinuesa CG, and Cyster JG, 2011.?Immunity?35:671.
3. Kitano M,?et al.?2011.?Immunity?34:961.
4. Baumjohann D,?et al.?2011.?J. Immunol.?187:2089.
5. Johnston RJ,?et al.?2009.?Science.?325:1006.
6. Tran TH,?et al.?2010.?Cancer Res.?70:1711.
Gene ID: 604 View all products for this Gene ID
UniProt: View information about Bcl-6 on UniProt.org

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