Purified anti-human Siglec-8 Antibody
- 产品名称：
- Purified anti-human Siglec-8 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 347102
- 产品应用：
- 347102
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Siglec-8 fused to human IgG Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Floyd H, et al. 2000. J. Biol. Chem. 275:861.
- Wen T, et al. 2014. J Immunol. 192:5481. PubMed
- Product Citations
-
- Kano G, et al. 2013. J Allergy Clin Immunol. 132:437. PubMed
- Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
- Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
- Meng X, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
- Arakawa S, et al. 2018. Allergol Int. 67S:S41. PubMed
- Wen T, et al. 2014. J Immunol. 192:5481. PubMed
- Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
- RRID
- AB_2239311 (BioLegend Cat. No. 347102)
- Structure
- A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain
- Distribution
-
Eosinophils, basophils, mast cells
- Function
- Cell adhesion, signal transduction
- Interaction
- Phosphatases containing a SH2 domain
- Bioactivity
- Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
- Cell Type
- Basophils, Eosinophils, Mast cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, Siglec Molecules
- Antigen References
-
1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317.
2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608.
3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.
- Gene ID
- 27181 View all products for this Gene ID
- UniProt
- View information about Siglec-8 on UniProt.org
