Purified anti-complement C3/C3b/iC3b Antibody
- 产品名称：
- Purified anti-complement C3/C3b/iC3b Antibody
- 产品类别：
- 抗体
- 产品编号：
- 846402
- 产品应用：
- 846402
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human iC3b coupled to sepharose 4B
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
FC, Direct ELISA - Verified
ELISA Detection, RIA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per ml.?For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 ?g per million cells in 100 ?l volume. For Direct ELISA, the suggested use of this reagent is 0.01 - 2.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: ELISA Detection1 and RIA2.
This antibody recognizes human C3, C3b and iC3b, and does not cross-react with C3d.
- Application References
(PubMed link indicates BioLegend citation) -
- Tosic L, et al. 1989. J. Immunol. Methods 120:241. (ELISA)
- Sokoloff MH, et al. 2000. Cancer Immunol. Immunother. 49:551. (RIA, FC)
- Kennedy AD, et al. 2003. Blood 101:1071. (FC)
- Lindorfer MA, et al. 2010. Blood 115:2283. (FC)
- Beurskens, et al. 2012. J. Immunol. 188:3532. (FC)
- RRID
- AB_2572175 (BioLegend Cat. No. 846402)
- Structure
- C3 is a 185 kD glycoprotein that is cleaved to generate a 176 kD protein fragment, C3b. iC3b is proteolytically derived from C3b and exists as a mixture of 176 and 174 kD proteins.
- Distribution
-
Extracellular, plasma membrane, and serum.
- Function
- C3b is involved in all three complement pathways and is essential for effective complement activation and presentation of antigens to cells of the adaptive immune system. iC3b is less active than C3b, but the target-bound protein can expand target-specific B-cell and T-cell populations by interacting with lymphoid and phagocytic cells.
- Interaction
- Immune cells interact with C3b and iC3b using CR1 and CR2/CR3, respectively.
- Ligand/Receptor
- C3b can bind to Factor B, Factor P, Factor H, Factor I, C5, DAF (CD55), MCP (CD46), and CR1 (CD35). iC3b interacts with CR2 (CD21) and CR3 (CD11b/CD18).
- Biology Area
- Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Antigen References
-
1. Lambris JD, et al. 1988. Immunol. Today 9:387-93.
- Gene ID
- 718 View all products for this Gene ID
- UniProt
- View information about C3/C3b/iC3b on UniProt.org
