Purified anti-CRISPR (CAS9) Antibody
- 产品名称：
- Purified anti-CRISPR (CAS9) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 844302
- 产品应用：
- 844302
- Verified Reactivity
- S. Pyogenes
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant S. pyogenes Cas9 Protein (N-terminal 250 amino acids) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICC - Quality tested
WB, IP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, the suggested working dilution is 1.0 - 10??g/ml. For western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- Usman WM, et al. 2018. Nat Commun. 9:278. PubMed
- Zafra MP, et al. 2018. Nat Biotechnol. 36:888. PubMed
- Smeenk L, et al. 2021. Cancer Discov. 11:2868. PubMed
- Jang HK, et al. 2021. Sci Adv. 7:. PubMed
- Geisinger JM, et al. 2020. Nucleic Acids Res. 48:9067. PubMed
- Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed
- Habib O, et al. 2022. Nucleic Acids Res. 50:1187. PubMed
- Siadous FA, et al. 2020. Autophagy. :1. PubMed
- Scheidmann MC, et al. 2022. Cancer Res. 82:681. PubMed
- Freitas GP, et al. 2021. Gene Ther. 28:748. PubMed
- Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed
- Henriksson J, et al. 2019. Cell. 176:882. PubMed
- RRID
- AB_2749904 (BioLegend Cat. No. 844302) AB_2565570 (BioLegend Cat. No. 844301)
- Structure
- The architectures of Cas9 enzymes define nucleic acid binding clefts to form a central channel where DNA substrates are bound.
- Function
- The Cas9 protein from S. pyogenes is part of the CRISPR/Cas9 system of acquired immunity that bacteria have evolved to destroy foreign DNA elements, such as bacteriophages.
- Biology Area
- Cell Biology, Neuroscience
- Antigen References
-
1. Mali P, et al. 2013. Science 339:823.
- Gene ID
- 901176 View all products for this Gene ID
- UniProt
- View information about CRISPR on UniProt.org
