PE anti-NFATc1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1836	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant protein of human NFATc1 amino acids 197-304.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

7A6 antibody detects endogenous human NFATC1 in Western blot with an approximate molecular weight of 100 - 120 kD (different isoforms) (Cat. No. 649602). The optimal concentration should be determined by titration for each individual assay of interest.

Clone 7A6 Intracellular Staining Procedure:

Prepare the fix/perm solution, consisting of 1% paraformaldehyde in 70% ethanol. Chill for at least one hour at -20°C.
Suspend cells in cell staining buffer and perform surface stain if necessary.
Centrifuge for 5 minutes at 1500 RPM and discard supernatant.
Resuspend cells in 500 ?L of fix/perm solution for up to 2 x 10^6 cells and gently vortex.
Incubate cells on ice for 30-60 minutes in the dark.
Add 2 mL PBS and centrifuge cells for 1500 RPM for 5 minutes.
Wash cells 1X with 2 mL cell staining buffer, centrifuge at 1500 RPM for 5 minutes, discard supernatant.
Adjust cell concentration to about 1 x 10^7 cells/mL, add 100 ?L (~ 1 x 10^6 cells) to a FACS tube that already contains the staining antibody and mix gently.
Incubate 15 minutes at room temperature.
Wash 2X with cell staining buffer as in step 6.
Resuspend cells in cell staining buffer and analyze.

Application References (PubMed link indicates BioLegend citation)

Timmerman LA, et al. 1997. J. Immunol. 159:2735. (IF)
Brandt C, et al. 2010. Cytometry A. 77:607. (FC)
Fan W, et al. 2012. Arthritis Rheum. 64:3715. PubMed

Product Citations

Jung IY, et al. 2022. Sci Transl Med. 14:eabn7336. PubMed
Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed

RRID

AB_2562546 (BioLegend Cat. No. 649605) AB_2562547 (BioLegend Cat. No. 649606)

Antigen Details

Structure: 943 amino acids with predicted molecular weight of 101 kD.
Distribution: Cytoplasm, Nucleus. NFATc1 is cytoplasmic in the phosphorylated form and nuclear after calcineurin-mediated dephosphorylation controlled activation.
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Zhao Q, et al. 2010. Int. J. Biochem. Cell Biol. 42:576.
2. Hoey T, et al. 1995. Immunity 2:461.
3. Northrop JP, et al. 1993. J. Biol. Chem. 268:2917.
4. Hogan PG, et al. 2003. Genes Dev. 17:2205.
5. Shaw KT, et al. 1995. P. Natl. Acad. Sci. USA 92:11205.
6. Serfling E, et al. 2007. Sci. STKE 138:pe42.
Gene ID: 4772 View all products for this Gene ID
UniProt: View information about NFATc1 on UniProt.org

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