Go-ChIP-Grade™ Purified anti-NFATc1 Antibody-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 996	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant protein of human NFATc1 amino acids 197-304.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ChIP - Quality tested
WB - Verified
ICC, ICFC - Reported in the literature, not verified in house?

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:100-1:2000?by volume. For Western blotting, the suggested use of this reagent is 1.0??g per ml. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

25 ?g, 100 ?g of Go-ChIP-Grade? Purified Antibody can be used for 4-50, 16-200 immunoprecipitations, respectively, at the recommended dilution.

7A6 antibody detects endogenous human NFATC1 in Western blot. There are 10 isoforms with a?predicted?MW of 39KD, 74kD, 76kD, 77kD (3), 88kD (2), 100kD, 101kD. With this antibody, observed MW bands range from 70 - 120 kD.?The optimal concentration should be determined by titration for each individual assay of interest.

Application References (PubMed link indicates BioLegend citation)

Timmerman LA, et al. 1997. J. Immunol. 159:2735. (IF)
Brandt C, et al. 2010. Cytometry A. 77:607. (FC)
Fan W, et al. 2012. Arthritis Rheum. 64:3715. PubMed

Product Citations

Zhong Y, et al. 2022. Nat Immunol. 23:122. PubMed

RRID

AB_2721595 (BioLegend Cat. No. 649607) AB_2721596 (BioLegend Cat. No. 649608)

Antigen Details

Structure: 943 amino acids with predicted molecular weight of 101kD. There are 10 isoforms with a predicted MW of 39kD, 74kD, 76kD, 77kD (3), 88kD (2), 100kD, 101kD.
Distribution: Cytoplasm, Nucleus. NFATc1 is cytoplasmic in the phosphorylated form and nuclear after calcineurin-mediated dephosphorylation controlled activation.
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Zhao Q, et al. 2010. Int. J. Biochem. Cell Biol. 42:576.
2. Hoey T, et al. 1995. Immunity 2:461.
3. Northrop JP, et al. 1993. J. Biol. Chem. 268:2917.
4. Hogan PG, et al. 2003. Genes Dev. 17:2205.
5. Shaw KT, et al. 1995. P. Natl. Acad. Sci. USA 92:11205.
6. Serfling E, et al. 2007. Sci. STKE 138:pe42.
Gene ID: 4772 View all products for this Gene ID
UniProt: View information about NFATc1 on UniProt.org

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