Purified anti-Cytoplasmic Dynein Intermediate Chai
- 产品名称：
- Purified anti-Cytoplasmic Dynein Intermediate Chai
- 产品类别：
- 抗体
- 产品编号：
- 904901
- 产品应用：
- 904901
- Verified Reactivity
- Vertebrates, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against purified bovine brain cytoplasmic dynein.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 ?g per ml (1:1000).?For immunocytochemistry, a concentration range of 4.0 - 10.0 μg/ml (1:100-1:250) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is effective in immunohistochemistry (IHC), immunoblotting and immunoprecipitation (IP).
*Predicted MW = 74 kD. This antibody is known not to react in squid and nematode.
Clone 74.1 reacts with all isoforms from both rat IC74 genes. The antigenic site is believed to be located in the conserved amino-terminal 60 amino acids. Clone 74.1 has been shown to inhibit the activity of cytoplasmic dynein.
- Application References
(PubMed link indicates BioLegend citation) -
- Miller MG, et al. 1999. Biol Reprod. 60:1047.
- Dillman JF, et al. 1996. Proc Natl Acad Sci USA. 93:141.
- Pfister KK, et al. 1996.J Biol Chem. 271:1687.
- Dillman JF, et al. 1994. J Cell Biol. 127:1671.
- Combs J, et al. 2006. PNAS. 103:14883.
- Chen JL, et al. 2005. J Cell Biol. 169:383. (IP) PubMed
- Burakov A, et al. 2008. Traffic. 9:472.
- Payne C, et al. 2003. J Cell Sci. 116:4727. (ICC) PubMed
- Semenova I, et al. 2014. Mol Biol Cell. 25:3119. (WB) PubMed
- Product Citations
-
- Dwivedi D, et al. 2019. J Cell Biol. 218:871. PubMed
- Payne C, et al. 2003. J Cell Sci. 116:4727-4738. PubMed
- Chen J, et al. 2005. J Cell Biol. 169:383-389. PubMed
- Nath S, et al. 2016. J Immunol. 197: 2090 - 2101. PubMed
- Sabblah TT, et al. 2018. Sci Rep. 1.540972222. PubMed
- Zhang J, et al. 2016. Sci Rep. 6:37050. PubMed
- Semenova I, et al. 2014. Mol Biol Cell. 25:3119-3132. PubMed
- RRID
- AB_2565044 (BioLegend Cat. No. 904901)
