Alexa Fluor® 594 anti-Cytoplasmic Dynein Inter
- 产品名称：
- Alexa Fluor® 594 anti-Cytoplasmic Dynein Inter
- 产品类别：
- 抗体
- 产品编号：
- 904903
- 产品应用：
- 904903
- Verified Reactivity
- Vertebrates, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against purified bovine brain cytoplasmic dynein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
This antibody is effective in immunohistochemistry (IHC), immunoblotting and immunoprecipitation (IP).
*Predicted MW = 74 kD. This antibody is known not to react in squid and nematode.
Clone 74.1 reacts with all isoforms from both rat IC74 genes. The antigenic site is believed to be located in the conserved amino-terminal 60 amino acids. Clone 74.1 has been shown to inhibit the activity of cytoplasmic dynein.
- Application References
(PubMed link indicates BioLegend citation) -
- Miller MG, et al. 1999. Biol Reprod. 60:1047.
- Dillman JF, et al. 1996. Proc Natl Acad Sci USA. 93:141.
- Pfister KK, et al. 1996.J Biol Chem. 271:1687.
- Dillman JF, et al. 1994. J Cell Biol. 127:1671.
- Combs J, et al. 2006. PNAS. 103:14883.
- Chen JL, et al. 2005. J Cell Biol. 169:383. (IP) PubMed
- Burakov A, et al. 2008. Traffic. 9:472.
- Payne C, et al. 2003. J Cell Sci. 116:4727. (ICC) PubMed
- Semenova I, et al. 2014. Mol Biol Cell. 25:3119. (WB) PubMed
- RRID
- AB_2810813 (BioLegend Cat. No. 904903) AB_2810814 (BioLegend Cat. No. 904904)
