Anti-CD171 (L1CAM) Antibody (Previously Covance ca
- 产品名称：
- Anti-CD171 (L1CAM) Antibody (Previously Covance ca
- 产品类别：
- 抗体
- 产品编号：
- 838101
- 产品应用：
- 838101
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against purified chick L1, and binds to the L1 cytoplasmic domain at about T1172 (adjacent to the YRSL sorting signal).
- Preparation
- Ascites
- Concentration
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw.
- Application
-
WB -?Quality tested
ICC, IP, IHC-P?- Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution for this reagent is 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain.?
- Application References
(PubMed link indicates BioLegend citation) -
- Kamiguchi H,?et al.?1998.?J. Neurosci.?18(14):5311-21.
- Lemmon V,?et al.?1989.?Neuron.?2(6):1597-603.
- Yip PM, Siu CH. 2001.?J. Neurochem.?76(5):1552-64.
- Schaefer AW, et. al. 2002. J. Cell Biol. 157:1223. (WB, IHC-P, IP)
- Beer S, et. al. 1999. J. Cell Sci. 112:2667. (IP)
- Itoh K, et. al. 2005. Mol. Cell Neurosci. 29:245. (WB, IP)
- Zheng XJ, et. al. 2005. Environ. Technol. 26:1073. (ICC)
- Chen SY, et. al. 2001. FASEB. J. 15:1649. (IHC-P) PubMed
- Itoh K, et. al. 2004. J. Cell Biol. 165:145. (WB)
- Cohen NR, et. al. 1998. Curr. Biol. 8:26. (WB)
- Product Citations
-
- Chen S, et al. 2001. FASEB J. 15:1649-1561. PubMed
- Hu J, et al. 2022. Cell Death Dis. 13:48. PubMed
- Kleene R, et al. 2021. J Neurochem. 157:1102. PubMed
- Girbes Minguez M, et al. 2020. FASEB J. 34:9869. PubMed
- RRID
- AB_2565388 (BioLegend Cat. No. 838101)
