Purified anti-human CD169 (Sialoadhesin, Siglec-1)
- 产品名称：
- Purified anti-human CD169 (Sialoadhesin, Siglec-1)
- 产品类别：
- 抗体
- 产品编号：
- 346002
- 产品应用：
- 346002
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Rhinovirus (HRV14) infected, monocyte derived-DCs
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting and inhibition of erythrocyte-rosetting with cells expressing CD169.
- Application References
(PubMed link indicates BioLegend citation) -
- Kirchberger S, et al. 2005. J. Immunol. 175:1145.
- Schrauf C, et al. 2009. J. Immunol. 183:4440.
- Product Citations
-
- Lempp FA, et al. 2021. Nature. 598:342. PubMed
- Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
- Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
- Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
- Michlmayr D, et al. 2020. Cell Reports. 31(4):107569. PubMed
- Gruber CN, et al. 2020. Cell. 183:982. PubMed
- Rai MA, et al. 2020. Retrovirology. 17:35. PubMed
- Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
- Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
- Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
- Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
- RRID
- AB_2189031 (BioLegend Cat. No. 346002)
- Structure
- Type I single membrane-spanning lectin, 1709 amino acids, 210 kD, belongs to the immunoglobulin superfamily
- Distribution
-
Macrophages, dendritic cells
- Function
- Adhesion molecule
- Interaction
- α-2,3-linked sialic acid
- Ligand/Receptor
- CD227, CD43 and CD206
- Bioactivity
- Mediates sialic-acid dependent binding to granulocytes, monocytes, NK, B and T cells.
- Cell Type
- B cells, Dendritic cells, Macrophages
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules, Siglec Molecules
- Antigen References
-
1. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
2. Varki A, et al. 2009. Glycoconj J. 26:231.
3. Rempel H, et al. 2008. PLoS One. 3:e1967.
4. Crocker PR, et al. 2001. Trends Immunol. 22:337.
5. Hartnell A, et al. 2001. Blood 97:288.
- Gene ID
- 6614 View all products for this Gene ID
- UniProt
- View information about CD169 on UniProt.org
