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Biotin anti-β-Amyloid, 1-38 Antibody (Previou
产品名称:
Biotin anti-β-Amyloid, 1-38 Antibody (Previou
产品类别:
抗体
产品编号:
808603
产品应用:
808603
[价格]
规格 价格 库存
50µL ¥ 5700 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ELISA, WB, IHC
ICC?- Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.

The optimal working dilution should be determined for each specific assay condition.
? WB: 1:1,000 - 1:2,000*
? IHC: 1:50 - 1:100
Tissue: Formalin-fixed Paraffin-embedded Tissues
Pre-treatment: 70% Formic Acid for 20 minutes at room temperature
Primary incubation: 24 hours at 2-4°C

? ELISA: Can be used as capture antibody in a sandwich ELISA with 4G8 detection (Ref Cat# 800707 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody, HRP Labeled, or Cat No. 800701 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody).
For capture antibody, recommended usage is between 1.0 and 10.0 ?g/ml.
Recommended blocking buffer is 0.5-1.0% BSA in PBS.
If using Cat No. 800707 for detection; recommended usage is between 0.25-1.0 ?g/ml.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), and ELISA.

*Expected MW: 4 kD

WB Positive Control: Alzheimer's Brain Lysate

IHC Positive Control: Alzheimer's Brain Tissue

This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform that ends at the 38th amino acid.

Application References

(PubMed link indicates BioLegend citation)
  1. Watts JC, et al. 2014. Proc. Natl. Acad. Sci. USA 28:10323. ((F)
  2. Giaccone G, et al. 2012. Acta. Neuropathol. 124:809. (IHC, IF, WB)
Product Citations
  1. Ma C, et al. 2021. Front Immunol. 11:582998. PubMed
RRID
AB_2564746 (BioLegend Cat. No. 808603)
Disclaimer

Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 1-38 on UniProt.org

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