Purified anti-human CD123 Antibody
- 产品名称：
- Purified anti-human CD123 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 306002
- 产品应用：
- 306002
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IL-3Rα transfected COS cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF? - Verified
IHC-F, IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2?and also paraformaldehyde fixed paraffin embedded tissue7.
- Application References
(PubMed link indicates BioLegend citation) -
- Sun Q, et al. 1996. Blood 87:83. (IP, WB)
- Herling M, et al. 2003. Blood 101:5007. (IHC)
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
- Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
- Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
- Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
- Product Citations
-
- Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
- Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
- Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
- Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
- Parackova Z, et al. 2020. Sci Rep. 0.759027778. PubMed
- Kremenovic M, et al. 2020. Cancers (Basel). 12:. PubMed
- Mann ER, et al. 2020. Sci Immunol. :5. PubMed
- Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
- Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
- Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
- Syrimi E, et al. 2021. iScience. 24:103215. PubMed
- O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
- Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
- Chen S, et al. 2010. Arch Dermatol Res. 302:113. PubMed
- Olin A, et al. 2018. Cell. 174:1277. PubMed
- Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
- Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
- Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
- Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
- Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed
- Martin-Gayo E, et al. 2010. Blood. 115:5366. PubMed
- Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
- Henrick BM, et al. 2021. Cell. . PubMed
- Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
- NULL, et al. 2022. Cell. 185:916. PubMed
- Liu SQ, et al. 2021. Cancer Immunol Res. 9:34. PubMed
- Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
- Saygin C, et al. 2021. Leukemia. 35:3406. PubMed
- Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
- Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
- Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
- RRID
- AB_314576 (BioLegend Cat. No. 306002)
- Structure
- Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
- Distribution
-
Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
- Function
- Hematopoietic cell proliferation, differentiation
- Ligand/Receptor
- IL-3
- Cell Type
- Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Miyajima A, et al. 1993. Blood 82:1960.
- Gene ID
- 3563 View all products for this Gene ID
- UniProt
- View information about CD123 on UniProt.org
