PE anti-human CD123 Antibody
- 产品名称：
- PE anti-human CD123 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 306006
- 产品应用：
- 306006
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 2100 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IL-3Rα transfected COS cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2?and also paraformaldehyde fixed paraffin embedded tissue7.
- Application References
(PubMed link indicates BioLegend citation) -
- Sun Q, et al. 1996. Blood 87:83. (IP, WB)
- Herling M, et al. 2003. Blood 101:5007. (IHC)
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
- Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
- Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
- Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
- Product Citations
-
- Woo MS, et al. 2021. iScience. 24(7):102752. PubMed
- Zaro BW, et al. 2019. J Immunol. 202:2737. PubMed
- Luchsinger LL, et al. 2020. Cell Stem Cell. 25(2):225-240. PubMed
- Randrianarison-Huetz V, et al. 2010. Blood. 115:2784. PubMed
- Han L, et al. 2012. PLoS One. 4:e7989. PubMed
- Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
- Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
- Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
- Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
- Sefik E, et al. 2021. Nat Biotechnol. . PubMed
- Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
- Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
- Felce JH, et al. 2018. Sci Signal. 11:eaat0756. PubMed
- Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
- Li M, et al. 2020. Nat Commun. 4051:11. PubMed
- Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
- RRID
- AB_314579 (BioLegend Cat. No. 306005) AB_314580 (BioLegend Cat. No. 306006)
- Structure
- Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
- Distribution
-
Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
- Function
- Hematopoietic cell proliferation, differentiation
- Ligand/Receptor
- IL-3
- Cell Type
- Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Miyajima A, et al. 1993. Blood 82:1960.
- Gene ID
- 3563 View all products for this Gene ID
- UniProt
- View information about CD123 on UniProt.org
