FITC anti-human CD123 Antibody
- 产品名称：
- FITC anti-human CD123 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 306013
- 产品应用：
- 306013
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IL-3Rα transfected COS cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2?and also paraformaldehyde fixed paraffin embedded tissue7.
- Application References
(PubMed link indicates BioLegend citation) -
- Sun Q, et al. 1996. Blood 87:83. (IP, WB)
- Herling M, et al. 2003. Blood 101:5007. (IHC)
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
- Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
- Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
- Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
- Product Citations
-
- Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
- Murayama G,et al. 2017. Arthritis Res Ther.. 10.1186/s13075-017-1441-7. PubMed
- Hagen SH, et al. 2020. Cell Rep. 33:108485. PubMed
- Bj?rklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
- Ishimori A, et al. 2016. Allergol Int. pii: S1323-8930(16)30105-8. PubMed
- Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
- Imai Y, et al. 2021. JID Innov. 1:100003. PubMed
- Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
- Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
- Shi B, et al. 2012. PLoS One. 7:e44900. PubMed
- Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
- Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
- Pritchard A, et al. 2014. PLoS One. 9:106501. PubMed
- Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
- Hagen SH, et al. 2021. STAR Protocols. 2(3):100641. PubMed
- Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
- Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
- Matsuyama H, et al. 2019. Sci Rep. 9:13181. PubMed
- Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
- Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
- Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
- RRID
- AB_755989 (BioLegend Cat. No. 306013) AB_2124259 (BioLegend Cat. No. 306014)
- Structure
- Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
- Distribution
-
Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
- Function
- Hematopoietic cell proliferation, differentiation
- Ligand/Receptor
- IL-3
- Cell Type
- Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Miyajima A, et al. 1993. Blood 82:1960.
- Gene ID
- 3563 View all products for this Gene ID
- UniProt
- View information about CD123 on UniProt.org
