Purified anti-Cytochrome c Antibody
- 产品名称：
- Purified anti-Cytochrome c Antibody
- 产品类别：
- 抗体
- 产品编号：
- 612301
- 产品应用：
- 612301
- Verified Reactivity
- Mouse, Rat, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat cyt c-OVA
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
- Application
-
IP - Quality tested
ICC - Verified
ICFC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by?immunoprecipitation. This antibody can be used at 2.0 - 4.0 ?g /1 x107?cell equivalents for immunoprecipitation. For Western blot application,?the suggested use of this reagent is 0.5 ?g per ml (1:1000 dilution).?For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106?cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.
- Application References
(PubMed link indicates BioLegend citation) -
- Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134.
- Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143.
- Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF)
- Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP)
- Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF)
- She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed
- McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
- Product Citations
-
- Wang Z, et al. 2020. Theranostics. 5.401388889. PubMed
- Kruppa AJ et al. 2018. Developmental cell. 44(4):484-499 . PubMed
- She P, et al. 2011. Am J Physiol Endocrinol Metab. 301:E49. PubMed
- McGuire K, et al. 2011. J Virol. 85:10806. PubMed
- Rana PS, et al. 2020. J Cell Sci. 133:00:00. PubMed
- Ryan TA, et al. 2020. EMBO J. 39:e102539. PubMed
- Ponsford AH, et al. 2020. Autophagy. :1. PubMed
- Cserép C et al. 2018. eNeuro. 5(1) pii: ENEURO. PubMed
- RRID
- AB_315774 (BioLegend Cat. No. 612301) AB_315775 (BioLegend Cat. No. 612302)
- Structure
- Heme binding domain; 15 kD
- Distribution
-
Mitochondrial intermembrane space
- Function
- Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
- Interaction
- Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Mitochondrial Markers
- Antigen References
-
1. Liu X, et al. 1996. Cell. 86:147.
2. Li P, et al. 1997. Cell. 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
- Gene ID
- 1355 View all products for this Gene ID
- UniProt
- View information about Cytochrome c on UniProt.org
