GMP FITC anti-Cytochrome c Antibody
- 产品名称：
- GMP FITC anti-Cytochrome c Antibody
- 产品类别：
- 抗体
- 产品编号：
- 260100
- 产品应用：
- 260100
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 2820 |
1 |
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat cyt c-OVA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- 200 ?g/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.
- Application References
(PubMed link indicates BioLegend citation) -
- Goshorn SC,?et al.?1991.?J. Biol. Chem.?266:2134.
- Jemmerson R,?et al.?1991.?Eur. J. Immunol.?21:143.
- Chandra D,?et al.?2002.?J. Biol. Chem.?277:50842. (IF)
- Semenkova L,?et al.?2003.?Eur. J. Biochem.?270:4388. (IP)
- Shih S-F,?et al.?2001.?J. Biol. Chem.?276:21870. (ICFC ICC IF)
- She P,?et al.?2011.?Am J. Physiol Endcorinol Metab.301:E49.?PubMed
- McGuire, KA.,?et al.?2011.?J. Virol?85:10806.?PubMed
- RRID
- AB_2922512 (BioLegend Cat. No. 260100)
- Disclaimer
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
- Structure
- Heme binding domain; 15 kD
- Distribution
-
Mitochondrial intermembrane space
- Function
- Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
- Interaction
- Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Mitochondrial Markers
- Antigen References
-
- Liu X,?et al.?1996.?Cell.?86:147.
- Li P,?et al.?1997.?Cell.?91:479.
- Zhang Z,?et al.?2003.?Gene?312:61.
- Ferguson H,?et al.?2003.?J. Biol. Chem.?278:45793.
- Gene ID
- 1355 View all products for this Gene ID
- UniProt
- View information about Cytochrome c on UniProt.org
