Alexa Fluor® 647 anti-Cytochrome c Antibody
- 产品名称：
- Alexa Fluor® 647 anti-Cytochrome c Antibody
- 产品类别：
- 抗体
- 产品编号：
- 612310
- 产品应用：
- 612310
- Verified Reactivity
- Mouse, Rat, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat cyt c-OVA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume.?For immunocytochemistry, a concentration range of 2.0?- 5.0?μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.
- Application References
(PubMed link indicates BioLegend citation) -
- Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134.
- Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143.
- Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF)
- Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP)
- Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF)
- She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed
- McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
- Product Citations
-
- Sekar G, et al. 2022. iScience. 25:105064. PubMed
- Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed
- Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
- Ramsey HE, et al. 2018. Cancer Discov. 8:1566. PubMed
- Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
- Olesinski EA, et al. 2021. STAR Protocols. 2(2):100461. PubMed
- Fraser C, et al. 2022. Nat Commun. 13:5789. PubMed
- Bhatt S, et al. 2020. Cancer Cell. 38(6):872-890.e6. PubMed
- Jenson J, et al. 2017. Elife. 10.7554/eLife.25541. PubMed
- Canedo EC, et al. 2021. JCI Insight. 6:. PubMed
- Potter DS, et al. 2021. Cell Death Dis. 12:741. PubMed
- Manzano-Mu?oz A, et al. 2021. Front Cell Dev Biol. 9:695225. PubMed
- Alcon C, et al. 2021. Cells. 10:. PubMed
- Victorino F, et al. 2021. eLife. 0.416666666666667. PubMed
- Dhimolea E, et al. 2021. Cancer Cell. 39:240. PubMed
- Liang Z, et al. 2021. J Immunol. 207:2039. PubMed
- RRID
- AB_2565241 (BioLegend Cat. No. 612310)
- Structure
- Heme binding domain; 15 kD
- Distribution
-
Mitochondrial intermembrane space
- Function
- Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
- Interaction
- Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Mitochondrial Markers
- Antigen References
-
1. Liu X, et al. 1996. Cell. 86:147.
2. Li P, et al. 1997. Cell. 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
- Gene ID
- 1355 View all products for this Gene ID
- UniProt
- View information about Cytochrome c on UniProt.org
