Go-ChIP-Grade™ Purified anti-Histone H4 Trim-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 996	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This monoclonal antibody was raised against a synthetic peptide conjugated to KLH containing trimethylated lysine 20 of human Histone H4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ChIP - Quality tested
IHC-P, WB - Verified?

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested dilution is?1:100 - 1:500. For Western blotting, the suggested use of this reagent is 0.5 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry (IHC) and Western blotting (WB).

Additional Product Notes

25 ?g and?100 ?g of Go-ChIP-Grade? Purified Antibody can be used for 2-5 or 10-20 immunoprecipitations, respectively, at the recommended dilutions.

Application References (PubMed link indicates BioLegend citation)

Botuyan MV, et al. 2006. Cell. 127:1361. (WB)

RRID

AB_2632896 (BioLegend Cat. No. 827703) AB_2632844 (BioLegend Cat. No. 827704)

Antigen Details

Structure: Histone proteins H3 and H4 bind to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution: Histone H4 is ubiquitously expressed in all cells.
Function: Transcription regulation, DNA repair, DNA replication, chromosomal stability, silencing of repetitive DNA and transposons.
Interaction: Various transcription factors.
Biology Area: Cancer Biomarkers, Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References: 1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.
2. Bhasin M,?et al. 2006. J. Comput. Biol. 13:102.
3. Nelson DM, et al. 2016. Genome Biol. 17:158.?
Gene ID: 8359 View all products for this Gene ID
UniProt: View information about Histone H4 Trimethyl on UniProt.org

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