Ultra-LEAF™ Purified anti-β-Amyloid, 1--上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 4032	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

WB - Quality tested
IHC-P - Verified
ICC, IHC-F, EM - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 ?g per mL.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.2 - 5.0 ?g/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS).

This antibody clone has been reported for use in immunohistochemistry of free-floating sections^2,13.

Application References (PubMed link indicates BioLegend citation)

Thakker DR,?et al. 2009.?Proc. Natl. Acad. Sci. USA.?106(11):4501-6.?(IHC)?PubMed
Oddo S,?et al. 2005.?Proc. Natl. Acad. Sci. USA.?102(8):3046-51.?(IHC-other)?PubMed
Herzig M,?et al. 2004.?Nat. Neuro.?7(9):954-959.?(WB)?PubMed
Zheng Y,?et al.?2012.?PLoS One?6:39035.?(IHC-F)?PubMed
Abramowksi D,?et al.?J Neurosci.?32:1273.?(WB)?PubMed
Forny-Germano L,?et al.?2014.?J. Neurosci.?34:13629.?(WB, IHC)?PubMed
Gowert NS,?et al.?2014.?PLoS One?2:e90523.?(ICC, EM)?PubMed
Sandoval-Hernandez A,?et al.?2015.?PLoS One. 10: 0145467.?(IHC-F)
Kumar R,?et al.?2016.?Brain.?139:174-92?(WB)
Miyamoto T,?et al.?2016. J. Biol. Chem. 291:1719-34.?(WB)
Saito S,?et al.?2017.?Acta Neuropathol. Commun.?5:26-9.?(IHC-P)?PubMed
Omata Y,?et al.?2016.?Aging (Albany NY)?8(3):427.?(IHC-P)?PubMed
Peng W,?et al.?2016.?Neurobiol. Dis.?93:215.?(IHC-other)?PubMed
Mandler M,?et al.?2015.?PLoS One.?e0115237.?(WB, IHC, ELISA)?PubMed

RRID

AB_2810700 (BioLegend Cat. No. 803023) AB_2810701 (BioLegend Cat. No. 803024)

Antigen Details

Structure

Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secr

Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function

The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

APP/β-Amyloid

Antigen References