Alexa Fluor® 488 anti-β-Amyloid, 1-16 Ant-上海雅吉生物科技有限公司

规格	价格	库存
200µL	￥ 8136	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Concentration

1 mg/mL

Storage & Handling

Store between 2-8°C.

Application

IHC-P -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 ?g/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Blue Laser (488 nm)

Application Notes

This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS).

This antibody clone has been reported for use in immunohistochemistry of free-floating sections^2,13.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Thakker DR,?et al. 2009.?Proc. Natl. Acad. Sci. USA.?106(11):4501-6.?(IHC)?PubMed
Oddo S,?et al. 2005.?Proc. Natl. Acad. Sci. USA.?102(8):3046-51.?(IHC-other)?PubMed
Herzig M,?et al. 2004.?Nat. Neuro.?7(9):954-959.?(WB)?PubMed
Zheng Y,?et al.?2012.?PLoS One?6:39035.?(IHC-F)?PubMed
Abramowksi D,?et al.?J Neurosci.?32:1273.?(WB)?PubMed
Forny-Germano L,?et al.?2014.?J. Neurosci.?34:13629.?(WB, IHC)?PubMed
Gowert NS,?et al.?2014.?PLoS One?2:e90523.?(ICC, EM)?PubMed
Sandoval-Hernández A,?et al.?2015.?PLoS One. 10: 0145467.?(IHC-F)
Kumar R,?et al.?2016.?Brain.?139:174-92?(WB)
Miyamoto T,?et al.?2016. J. Biol. Chem. 291:1719-34.?(WB)
Saito S,?et al.?2017.?Acta Neuropathol. Commun.?5:26-9.?(IHC-P)?PubMed
Omata Y,?et al.?2016.?Aging (Albany NY)?8(3):427.?(IHC-P)?PubMed
Peng W,?et al.?2016.?Neurobiol. Dis.?93:215.?(IHC-other)?PubMed
Mandler M,?et al.?2015.?PLoS One.?e0115237.?(WB, IHC, ELISA)?PubMed

Product Citations

Lee JH, et al. 2022. EBioMedicine. 77:103903. PubMed
Cramer PE et al. 2017. Neurobiology of aging. 64:92-106 . PubMed
Paolicelli RC et al. 2017. Neuron. 95(2):297-308 . PubMed
Fujita K, et al. 2016. Sci Rep. 6:31895. PubMed
Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
McAlpine CS, et al. 2021. Nature. 595:701. PubMed
Paulus A, et al. 2021. Int J Mol Sci. 22:. PubMed
Abe Y, et al. 2020. Acta Neuropathol Commun. 8:67. PubMed
Chen WT, et al. 2020. Cell. 182(4):976-991.e19. PubMed

RRID

AB_2564765 (BioLegend Cat. No. 803013)

Antigen Details

Structure

Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.

Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function

The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

APP/β-Amyloid

Antigen References