Pacific Blue™ anti-mouse CD19 Antibody
- 产品名称：
- Pacific Blue™ anti-mouse CD19 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 115526
- 产品应用：
- 115526
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD19-expressing K562 human erythroleukemia cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence7.
- Application References
(PubMed link indicates BioLegend citation) -
- Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
- Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
- Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
- Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
- Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
- Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
- Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
- Olive V, et al. 2013. Elife. 2:822. PubMed
- Miyai T, et al. 2014. PNAS. 111:11780. PubMed
- Product Citations
-
- Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
- Schneider C, et al. 2018. Cell. 174:271. PubMed
- Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
- Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
- Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
- Dema B, et al. 2014. J Exp Med. 211:2159. PubMed
- Dow L, et al. 2014. PLoS One. 9:95236. PubMed
- Saravia J, et al. 2015. PLoS Pathog. 11: e1005217. PubMed
- Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
- Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
- Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
- Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
- Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
- Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed
- Lang V, et al. 2021. Elife. 10:. PubMed
- Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
- Haso W, et al. 2013. Blood. 121:1165. PubMed
- Chen RJ, et al. 2022. iScience. 25:105595. PubMed
- Minguet S, et al. 2017. Nat Immunol. 18:1150. PubMed
- Han S, et al. 2016. Arthritis Res Ther. 0.975. PubMed
- Kajikawa A, et al. 2015. PLoS One. 10: e0141713. PubMed
- Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
- Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
- O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
- Ding Y, et al. 2013. J Immunol. 191:1614. PubMed
- Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
- Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
- Molgora M, et al. 2020. Cell. 182:886. PubMed
- Korin B, et al. 2020. Sleep. :43. PubMed
- Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
- Pfeil J, et al. 2020. Front Immunol. 11:529035. PubMed
- Smith LK, et al. 2021. Elife. 10:. PubMed
- Fiala G, et al. 2015. J Exp Med. 212: 1693-1708. PubMed
- Chen Z, et al. 2020. J Immunol. 204:335. PubMed
- Call M, et al. 2009. J Immunol. 182:6342. PubMed
- RRID
- AB_493341 (BioLegend Cat. No. 115526) AB_439718 (BioLegend Cat. No. 115523)
- Structure
- Ig superfamily, associates with CD21 and CD81, 95 kD
- Distribution
-
Pro-B cells to mature B cells (during development), follicular dendritic cells
- Function
- Modulates B cell activation and differentiation
- Ligand/Receptor
- CD21, CD81, Leu-13
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
- Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
