Alexa Fluor® 700 anti-mouse CD19 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-mouse CD19 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 115527
- 产品应用：
- 115527
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD19-expressing K562 human erythroleukemia cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence7.
- Application References
(PubMed link indicates BioLegend citation) -
- Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
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- Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
- Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
- Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
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- Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
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- Olive V, et al. 2013. Elife. 2:822. PubMed
- Miyai T, et al. 2014. PNAS. 111:11780. PubMed
- Product Citations
-
- Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
- Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
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- Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed
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- Li J, et al. 2010. J Immunol. 184:6142. PubMed
- Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
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- Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
- Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
- Perry D, et al. 2012. J Immunol. 189:793. PubMed
- Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
- Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
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- Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
- Aldrich AL, et al. 2021. The Journal of Immunology. 206(4):751-765. PubMed
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- Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
- Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
- Vogel AB, et al. 2021. Nature. 592:283. PubMed
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- Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
- Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
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- Matsuda T, et al. 2022. iScience. 25:105324. PubMed
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- Kalafati L, et al. 2020. Cell. 183:771. PubMed
- Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed
- Vijay R, et al. 2021. J Exp Med. 218:. PubMed
- Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
- Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
- Bennion BG, et al. 2019. J Virol. 93. PubMed
- Rinaldi S, et al. 2021. PLoS Pathog. 17:e1009533. PubMed
- Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
- Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
- Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed
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- Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
- RRID
- AB_493734 (BioLegend Cat. No. 115527) AB_493735 (BioLegend Cat. No. 115528)
- Structure
- Ig superfamily, associates with CD21 and CD81, 95 kD
- Distribution
-
Pro-B cells to mature B cells (during development), follicular dendritic cells
- Function
- Modulates B cell activation and differentiation
- Ligand/Receptor
- CD21, CD81, Leu-13
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
- Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
