Alexa Fluor® 647 anti-mouse CD19 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD19 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 115525
- 产品应用：
- 115525
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD19-expressing K562 human erythroleukemia cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. For immunohistochemistry, a concentration range of 5.0?- 10??g/ml is suggested.?It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence7.
- Application References
(PubMed link indicates BioLegend citation) -
- Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
- Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
- Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
- Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
- Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
- Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
- Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
- Olive V, et al. 2013. Elife. 2:822. PubMed
- Miyai T, et al. 2014. PNAS. 111:11780. PubMed
- Product Citations
-
- Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
- Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
- Plzakova L, et al. 2014. Microb Pathog. 75:49. PubMed
- Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
- Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
- Gilleron J et al. 2018. Cell reports. 25(12):3329-3341 . PubMed
- Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
- Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
- Kovács B, et al. 2020. Front Immunol. 2.508333333. PubMed
- Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
- Krollmann C, et al. 2022. STAR Protoc. 3:101309. PubMed
- Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
- Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
- Plzakova L, et al. 2015. PLoS One. 10: 0132571. PubMed
- Lindner J, et al. 2014. J Immunol. 192:4483. PubMed
- Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
- Gralinski L, et al. 2015. PLoS Genet. 11: e1005504. PubMed
- RRID
- AB_493340 (BioLegend Cat. No. 115525) AB_389329 (BioLegend Cat. No. 115522)
- Structure
- Ig superfamily, associates with CD21 and CD81, 95 kD
- Distribution
-
Pro-B cells to mature B cells (during development), follicular dendritic cells
- Function
- Modulates B cell activation and differentiation
- Ligand/Receptor
- CD21, CD81, Leu-13
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
- Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
