Alexa Fluor® 488 anti-mouse CD19 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-mouse CD19 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 115524
- 产品应用：
- 115524
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD19-expressing K562 human erythroleukemia cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence7.
- Application References
(PubMed link indicates BioLegend citation) -
- Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
- Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
- Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
- Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
- Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
- Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
- Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
- Olive V, et al. 2013. Elife. 2:822. PubMed
- Miyai T, et al. 2014. PNAS. 111:11780. PubMed
- Product Citations
-
- Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
- Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
- Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
- Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
- Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
- Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
- Loh Z, et al. 2020. PLOS Pathogens. 16(7):e1008651. PubMed
- Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
- Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed
- Collado J, et al. 2013. Eur J Immunol. 43:2273. PubMed
- Wang J, et al. 2011. Am J Pathol. 178:2168. PubMed
- Zhu K, et al. 2022. EMBO Rep. 23:e54499. PubMed
- Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
- Seeley-Fallen M, et al. 2014. Proc Natl Acad Sci U S A. 111:9881. PubMed
- Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
- Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
- Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
- Song W, et al. 2022. Immunity. 55:290. PubMed
- RRID
- AB_493339 (BioLegend Cat. No. 115524) AB_389307 (BioLegend Cat. No. 115521)
- Structure
- Ig superfamily, associates with CD21 and CD81, 95 kD
- Distribution
-
Pro-B cells to mature B cells (during development), follicular dendritic cells
- Function
- Modulates B cell activation and differentiation
- Ligand/Receptor
- CD21, CD81, Leu-13
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
- Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
