Ultra-LEAF™ Purified anti-human MICA/MICB An-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3240	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-P, IHC-F, IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemistry^2,3,5 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation⁷, and blocking^2,3 of MIC mediated cytotoxicity. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 320919 & 320120).

Application References (PubMed link indicates BioLegend citation)

Groh V, et al. 1999. Science 279:1737.
Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879.
Groh V, et al. 2001. Nature Immunol. 2:255.
Li Z, et al. 2000. Immunogenetics 51:246.
Park EJ, et al. 2003. J. Immunol. 171:4131.
Jinushi M, et al. 2003. J. Immunol. 171:5423.
Wu J, et al. 2003. J. Immunol. 170:4196.

RRID

AB_2814175 (BioLegend Cat. No. 320919) AB_2814176 (BioLegend Cat. No. 320920)

Antigen Details

Structure: 65-75 kD glycoprotein, MHC class I-like transmembrane molecules that do not associate ?2-microglobulin, highly polymorphic
Distribution: Intestinal epithelium, epithelial tumors, endothelial cells, fibroblasts, INF-a-stimulated dendritic cells
Function: Activation of NK cells, subset of CD8⁺ α/? T cells and γ/δ T cells, suppression of T cell proliferation
Ligand/Receptor: NKG2D
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Fibroblasts
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Groh V, et al. 1996. Proc. Natl. Acad. Sci. USA. 93:12445.
2. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879.
3. Jinushi M, et al. 2003. J. Immunol. 170:1249.
4. Kriegeskorte AK, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:11805.
5. Groh V, et al. 1999. Science 279:1737.
Gene ID: 100507436 View all products for this Gene ID 4277 View all products for this Gene ID
UniProt: View information about MICA MICB on UniProt.org

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